Alteration of Gene Expression

Reverse genetics needs efficient tools to alter gene expression. FGC provides close to 200,000 clones for genetic manipulation. These include all Sigma's TRC1 shRNA clones for human and mouse for knock-down of expression and Open Biosystems' human ORF clones for over-expression. ORF clones also come handy as probes for in situ hybridization or northerns.

I human shRNAs I mouse shRNAs I

shRNA clones come in lentivirus vectors for efficient infection of many cell types, including non-dividing ones. On average, we provide 4-5 different clones per gene. In many cases, the different clones targeting the same gene provide different knock-down efficiencies and help to control off-target effects. Approximately 75% of all human and mouse refseq genes are targeted.

       The complete list of shRNA clones is available on our Downloads page . Detailed information about the TRC1 clones is available on Sigma's  RNAi webpage.

I human ORF clones I

The Open Biosystems ORF library provides approximately 13,000 clones for expression of one open reading frame per gene. These clones are most frequently used for over-expression of genes but can also be used as probes. ORF clones contain the Gateway sytem  for hassle-free transfer into any kind of expression vector.

       The list of available ORF clones is available on our Downloads page. Detailed information about the ORF clones is available on Open Biosystem's ORF webpage.